NADPH Oxidase 4 Antikörper

Produktnummer ABIN5692899

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NADPH Oxidase 4 in WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Human und Affe und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für NADPH Oxidase 4 Antikörper (ABIN5692899)

Target: NADPH Oxidase 4 (NOX4)

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NADPH Oxidase 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-NADPH Oxidase 4 Antikörper

Verwendungszweck: Anti-NADPH oxidase 4/NOX4 Antibody Picoband®

Sequenz: ILNTLLDDWK PYKLRRLYFI WVCRDIQSFR WFADLL

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NADPH oxidase 4/NOX4 Antibody Picoband® (ABIN5692899). Tested in IF, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NADPH oxidase 4, which shares 97.2% amino acid (aa) sequence identity with both mouse and rat NADPH oxidase 4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
1. Ago, T., Kitazono, T., Ooboshi, H., Iyama, T., Han, Y. H., Takada, J., Wakisaka, M., Ibayashi, S., Utsumi, H., Iida, M. Nox4 as the major catalytic component of an endothelial NAD(P)H oxidase. Circulation 109: 227-233, 2004. 2. Geiszt, M., Kopp, J. B., Varnai, P., Leto, T. L. Identification of Renox, an NAD(P)H oxidase in kidney. Proc. Nat. Acad. Sci. 97: 8010-8014, 2000. 3. Shiose, A., Kuroda, J., Tsuruya, K., Hirai, M., Hirakata, H., Naito, S., Hattori, M., Sakaki Y., Sumimoto, H. A novel superoxide-producing NAD(P)H oxidase in kidney. J. Biol. Chem. 276: 1417-1423, 2001.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-NADPH Oxidase 4 Antikörper (ABIN5692899)

Liu, Hong, Li, Ren, Wang, Xu, Shi, Xu: "A Cross Talk Between BRG1 and Males Absent on the First Contributes to Reactive Oxygen Species Production in a Mouse Model of Nonalcoholic Steatohepatitis." in: Antioxidants & redox signaling, (2018) (PubMed).

Meyer, Fredette, Daniel, Sharma, Amann, Arterburn, Barton, Prossnitz: "Obligatory role for GPER in cardiovascular aging and disease." in: Science signaling, Vol. 9, Issue 452, pp. ra105, (2017) (PubMed).

Details zu NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Andere Bezeichnung: NOX4

Hintergrund:

Synonyms: NADPH oxidase 4, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD (P)H-oxidase, NOX4, RENOX

Tissue Specificity: Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

Background: NADPH oxidase 4 is an enzyme that in humans is encoded by the NOX4 gene, and is a member of the NOX family of NADPH oxidases. This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 65 kDa

Gen-ID: 50507

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration