Cathepsin D Antikörper (AA 161-358)

Produktnummer ABIN5692809

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Cathepsin D in WB, ELISA, ICC und IF. Er zeigt eine Reaktivität gegenüber Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für Cathepsin D Antikörper (AA 161-358) (ABIN5692809)

Target: Cathepsin D (CTSD)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cathepsin D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-Cathepsin D Antikörper

Bindungsspezifität: AA 161-358

Verwendungszweck: Anti-Cathepsin D/Ctsd Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Cathepsin D/Ctsd Antibody Picoband® (ABIN5692809). Tested in ELISA, IF, ICC, and WB applications. This antibody reacts with Mouse and Rat, offering an essential tool for scientific applications such as cell lysate analysis and breast adenocarcinoma cell line research. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived mouse Cathepsin D recombinant protein (Position: K161-Q358).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot,0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
ELISA,0.1-0.5 μg/mL
1. Faust PL, Kornfeld S, Chirgwin JM (Sep 1985). "Cloning and sequence analysis of cDNA for human cathepsin D". Proc Natl Acad Sci U S A 82 (15): 4910-4. 2. Wolf M, Clark-Lewis I, Buri C, Langen H, Lis M, Mazzucchelli L (April 2003). "Cathepsin D specifically cleaves the chemokines macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, and SLC that are expressed in human breast cancer". Am. J. Pathol. 162 (4): 1183-90.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Cathepsin D Antikörper (ABIN5692809)

Zheng, Li, Wang, Wang, Liao, Hu, Fan, Kang, Zeng, Wu, Wu, Zhang, Wang, He et al.: "Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage ..." in: Autophagy, Vol. 12, Issue 9, pp. 1593-613, (2017) (PubMed).

Details zu Cathepsin D

Target: Cathepsin D (CTSD)

Andere Bezeichnung: Ctsd

Hintergrund:

Synonyms: Cathepsin D, Ctsd

Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in signal peptide and a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation of proteins, cell invasion, and apoptosis, playing a significant role in cell signaling technology. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer's disease and it has been considered as a breast cancer tumor marker.

Molekulargewicht: 45 kDa

Gen-ID: 13033

UniProt: P18242

Pathways: Peptide Hormone Metabolism