ICAM-3/CD50 Antikörper (Domain 1, Domain 2)

Produktnummer ABIN5691103

Der Maus Monoklonal Anti-ICAM-3/CD50-Antikörper wurde für FACS, WB, ELISA, IP, ICC und IHC (fro) validiert. Er ist geeignet, ICAM-3/CD50 in Proben von Human zu detektieren.

Kurzübersicht für ICAM-3/CD50 Antikörper (Domain 1, Domain 2) (ABIN5691103)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM-3/CD50 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: JA4

Produktdetails anti-ICAM-3/CD50 Antikörper

Bindungsspezifität: Domain 1, Domain 2

Spezifität: JA4 recognizes a linear epitope in domains 1 and 2 of ICAM-3.

Immunogen: JA4 is a mouse monoclonal IgG1/k antibody derived by fusion of mouse Ag8.653 cells with spleen cells from a BALB/c mouse immunized with an ICAM-3/HEK transfectant.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: JA4 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C, or in small aliquots at -20°C.

Details zu ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Andere Bezeichnung: ICAM-3

Hintergrund: Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a type I transmembrane glycoprotein, which normally contain 2-9 immunoglobulin-like C2-type domains, and binds to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 has previously been implicated in apoptotic cell clearance, although its precise role in the clearance process is ill defined.