Tryptophan Hydroxylase 1 Antikörper

Produktnummer ABIN5690709

Dieser Schaf Polyklonal Antikörper detektiert spezifisch Tryptophan Hydroxylase 1 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für Tryptophan Hydroxylase 1 Antikörper (ABIN5690709)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reaktivität: Ratte

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Tryptophan Hydroxylase 1 Antikörper

Aufreinigung: Affinity Purified

Immunogen: TPH polyclonal antibody was raised against recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E.

Anwendungsinformationen

Applikationshinweise: Tryptophan Hydroxylase antibody can be used for specific immunolabeling of the ~55k TPH protein in Western blots of human dorsal Raphe nucleus. Applications include Dot Blots (DB) Immunohistochemistry (IHC) and Western Blots (WB). The antibody has been used successfully with rat and human brain tissues in blot immunolabeling and immunohistochemical procedures. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Details zu Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Andere Bezeichnung: Tryptophan Hydroxylase

Hintergrund: Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis..

Molekulargewicht: 55 kDa

Gen-ID: 100009100

UniProt: P17290

Pathways: Feeding Behaviour