APAF1 Antikörper (AA 1-464)

Produktnummer ABIN5690621

Der Ratte Monoklonal anti-APAF1 Antikörper (Klon 2E12) (ABIN5690621) detektiert spezifisch APAF1 in WB, IHC, ELISA, IP und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für APAF1 Antikörper (AA 1-464) (ABIN5690621)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 2E12

Produktdetails anti-APAF1 Antikörper

Bindungsspezifität: AA 1-464

Spezifität: Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.

Reinheit: > 95% (SDS-PAGE)

Immunogen: Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: (paraffin sections, 10 μg/mL) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al., (2000)) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for at least 1 year after receipt when stored at -20°C.

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: Apaf-1

Hintergrund: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Gen-ID: 317

UniProt: O14727

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity