CCL3 Antikörper (Biotin)

Produktnummer ABIN5690517

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch CCL3 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CCL3 Antikörper (Biotin) (ABIN5690517)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CCL3 Antikörper (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1a (Human MIP-1 alpha ). Human MIP-1a specific antibody was purified by affinity chromatography and then biotinylated.

Anwendungsinformationen

Applikationshinweise: ELISA:
Direct:
To detect hMIP-1a by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a.

Sandwich
To detect hMIP-1a by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human MIP-1a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a.

Western Blot:
To detect hMIP-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: MIP-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Andere Bezeichnung: MIP-1a

Hintergrund: Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

Gen-ID: 6348

UniProt: P10147

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagie