PAG1 Antikörper (AA 412-424)

Produktnummer ABIN5689872

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PAG1 in WB, IP und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PAG1 Antikörper (AA 412-424) (ABIN5689872)

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-PAG1 Antikörper

Bindungsspezifität: AA 412-424

Spezifität: Recognizes mouse, rat and human PAG/Cbp.

Aufreinigung: Antigen affinity purified.

Immunogen: Synthetic peptide corresponding to aa 412-424 of the N-terminal rat PAG including an additional N-terminal Cys residue.

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: (1:50). Immunoprecipitation: (5 μg/sample) . Western Blot: (1:1,000) . Optimal conditions must be determined individually for each application.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for at least 1 year after receipt when stored at -20°C.

Details zu PAG1

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Andere Bezeichnung: PAG/Cbp

Hintergrund: PAG [Cbp] is a ubiquitously expressed transmembrane adapter protein. Depending on its phosphorylation status, it appears as diffuse band(s) of 80-90  kDa by SDS-PAGE, with phosphorylation leading to a shift towards a higher apparent molecular mass. Tyrosine phosphorylated PAG recruits Csk to the membrane and negatively regulates Src family kinases via Csk-mediated phosphorylation. Upon T cell receptor engagement, PAG becomes dephosphorylated, thereby displacing Csk from the membrane and enabling the activation of the Src kinases Fyn and Lck. PAG negatively regulates TCR (T cell antigen receptor)-mediated signaling in T cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. It promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins and may be involved in cell adhesion signaling.

Gen-ID: 64019

UniProt: Q9JM80

Pathways: p53 Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, CXCR4-mediated Signaling Events