GFI1B Antikörper (AA 322-326)

Produktnummer ABIN5689567

Der Kaninchen Polyklonal anti-GFI1B Antikörper wird verwendet zum Nachweis von GFI1B in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für GFI1B Antikörper (AA 322-326) (ABIN5689567)

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFI1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GFI1B Antikörper

Bindungsspezifität: AA 322-326

Spezifität: This antibody detects endogenous level of total GFI1b protein.

Aufreinigung: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: GFI1b antibody  was raised against a peptide sequence around aa.322~326 (H-R-E-S-Q) derived from Human GFI1b.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:500~1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store antibody at -20°C for up to one year.

Details zu GFI1B

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Andere Bezeichnung: GFI1b

Hintergrund: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context, represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1, GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription, as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

Molekulargewicht: 38 kDa

Gen-ID: 8328

NCBI Accession: NP_001128503

UniProt: Q5VTD9

Pathways: Cellular Response to Molecule of Bacterial Origin