IL-7 Antikörper (Biotin)

Produktnummer ABIN5689149

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch IL-7 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für IL-7 Antikörper (Biotin) (ABIN5689149)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-7 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-IL-7 Antikörper (Biotin)

Aufreinigung: Anti-mIL-7 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-7 (murine IL-7).

Anwendungsinformationen

Applikationshinweise: ELISA:

To detect mIL-7 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-7.

Sandwich:

To detect mIL-7 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-7 (XP-5198) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-7.

Western Blot:
To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IL-7 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: IL-7

Hintergrund: Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4 and IL-6, suggesting that IL-7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. In myeloid lineage cells, IL-7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Gen-ID: 16196

UniProt: P10168

Pathways: JAK-STAT Signalweg