CXCL12 Antikörper

Produktnummer ABIN5689108

Der Kaninchen Polyklonal Anti-CXCL12-Antikörper wurde für WB, ELISA und Neut validiert. Er ist geeignet, CXCL12 in Proben von Human zu detektieren.

Kurzübersicht für CXCL12 Antikörper (ABIN5689108)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-CXCL12 Antikörper

Aufreinigung: Anti-hSDF-1-alpha specific antibody was purified by affinity chromatography employing immobilized hSDF-1-alpha matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSDF-1-alpha (human Stromal Cell-Derived Factor-1-alpha).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1α, (100 ng/mL), a concentration of 2-4 μ,g/mL of this antibody is required.

ELISA:

To detect hSDF-1-alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1-alpha.

Sandwich:

To detect hSDF-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human SDF-1α, (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1α,.

Western Blot:
To detect hSDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: SDF-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: SDF-1a

Gen-ID: 6387

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling