IL-11 Antikörper (Biotin)

Produktnummer ABIN5689027

Der Kaninchen Polyklonal Anti-IL-11-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, IL-11 in Proben von Human zu detektieren.

Kurzübersicht für IL-11 Antikörper (Biotin) (ABIN5689027)

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-11 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-IL-11 Antikörper (Biotin)

Aufreinigung: Anti-hIL-11 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-11 (human IL-11).

Anwendungsinformationen

Applikationshinweise: ELISA:

To detect hIL-11 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-11.

Sandwich

To detect hIL-11 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-11 (XP-5164) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-11.

Western Blot:

To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IL-11 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Andere Bezeichnung: IL-11

Hintergrund: Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.

Gen-ID: 3589

UniProt: P20809

Pathways: JAK-STAT Signalweg, Negative Regulation of Hormone Secretion