GM-CSF Antikörper

Produktnummer ABIN5689006

Der Kaninchen Polyklonal anti-GM-CSF Antikörper wird verwendet zum Nachweis von GM-CSF in Proben von Human. Er wurde validiert für WB, ELISA und Neut.

Kurzübersicht für GM-CSF Antikörper (ABIN5689006)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GM-CSF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-GM-CSF Antikörper

Aufreinigung: Anti-hGM-CSF specific antibody was purified by affinity chromatography employing immobilized hGM-CSF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF (human Granulocyte Macrophage Colony Stimulating Factor).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hGM-CSF (1.0 ng/mL), a concentration of 0.30-0.50 μ,g/mL of this antibody is required.

ELISA:

To detect hGM-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGM-CSF.

Sandwich: To detect hGM-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GM-CSF (XP-5144Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF.

Western Blot:
To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: GM-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: GM-CSF

Gen-ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin