CAMK2A Antikörper (pThr286)

Produktnummer ABIN5688927

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CAMK2A in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Xenopus laevis.

Kurzübersicht für CAMK2A Antikörper (pThr286) (ABIN5688927)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAMK2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CAMK2A Antikörper

Bindungsspezifität: pThr286

Spezifität: CaM Kinase II antibody is specific for a 50 kDa and b 60 kDa CaM Kinase II phosphorylated at Thr286.

Aufreinigung: Affinity Purified

Immunogen: CaM Kinase II (Thr286) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.

Anwendungsinformationen

Applikationshinweise: Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody. Applications include Dot Blot (DB) and Western Blot (WB). Dot Blots and Western Blots performed with each lot and working dilutions internally tested under ideal conditions for DB and WB were 1:1000. Suitability for Immunohistochemistry (IHC) has not been determined. Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Details zu CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Andere Bezeichnung: CaM Kinase II

Hintergrund: Calcium/calmodulin kinase II (CaM Kinase II) is multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.

Molekulargewicht: 50/60 kDa

Gen-ID: 25400

UniProt: P11275

Pathways: WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction