ERK1/2 Antikörper (pThr202, pTyr204)

Produktnummer ABIN5688926

Dieses Anti-ERK1/2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ERK1/2 in WB und IHC. Geeignet für Human, Maus, Ratte und Xenopus laevis.

Kurzübersicht für ERK1/2 Antikörper (pThr202, pTyr204) (ABIN5688926)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1/2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ERK1/2 Antikörper

Bindungsspezifität: pThr202, pTyr204

Spezifität: ERK1/2 antibody is specific for the 42/44 k ERK/MAPK protein phosphorylated at Thr202 and Tyr204..

Aufreinigung: Affinity Purified

Immunogen: ERK/MAPK (Thr202/Tyr204) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.

Anwendungsinformationen

Applikationshinweise: Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.

Details zu ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Andere Bezeichnung: ERK1 / 2

Hintergrund: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.

Molekulargewicht: 42/44 kDa

Gen-ID: 116590

UniProt: P63086