IL-7 Antikörper

Produktnummer ABIN5688700

Der Maus Monoklonal Anti-IL-7-Antikörper wurde für WB, ELISA und Neut validiert. Er ist geeignet, IL-7 in Proben von Human zu detektieren.

Kurzübersicht für IL-7 Antikörper (ABIN5688700)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Klon: NYR-hi7

Produktdetails anti-IL-7 Antikörper

Aufreinigung: This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.

Immunogen: The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-7 as the immunizing antigen.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: IL-7 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: IL-7

Hintergrund: Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4 and IL-6, suggesting that IL-7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. In myeloid lineage cells, IL-7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Gen-ID: 3574

UniProt: P13232

Pathways: JAK-STAT Signalweg