Thrombopoietin Antikörper

Produktnummer ABIN5687619

Produktdetails anti-Thrombopoietin Antikörper

Target: Thrombopoietin (THPO)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Thrombopoietin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Aufreinigung: Anti-hTPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5 ng/mL), a concentration of 4.2 - 6.3 μ,g/mL of this antibody is required.

ELISA:

To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTPO.

Sandwich:

To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TPO (XP-5288Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

Western Blot:
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu Thrombopoietin

Target: Thrombopoietin (THPO)

Andere Bezeichnung: Thrombopoietin (TPO) (THPO Produkte)

Synonyme: MGDF antikoerper, MKCSF antikoerper, ML antikoerper, MPLLG antikoerper, THCYT1 antikoerper, TPO antikoerper, Mgdf antikoerper, Ml antikoerper, Mpllg antikoerper, Tpo antikoerper, Tpo1 antikoerper, Tpo2 antikoerper, Tpo3 antikoerper, Tpo4 antikoerper, tpo antikoerper, LOC100231762 antikoerper, thrombopoietin antikoerper, THPO antikoerper, Thpo antikoerper, thpo antikoerper

Hintergrund: Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gen-ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signalweg, Hormone Activity