C5 Antikörper (FITC)
Kurzübersicht für C5 Antikörper (FITC) (ABIN568577)
Target
Alle C5 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- The reactivity of the antiserum is restricted to C5. In immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum. The antiserum does not cross react with any other component of human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
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Produktmerkmale
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Absorption / Emission: 492 nm / 515 nm
Molar Ratio: Fluorescein/IgG ~1.4 -
Aufreinigung
- The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
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Immunogen
- C5 protein is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization
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Isotyp
- IgG
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Rekonstitution
- Restore by adding 1 mL of sterile distilled water.
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Konzentration
- 10 mg/mL
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Buffer
- PBS, pH 7.2 without preservatives.
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Konservierungsmittel
- Without preservative
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Handhabung
- Avoid Repeated thawing and freezing.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- C5 (Complement Component 5 (C5))
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Andere Bezeichnung
- Complement C5
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Hintergrund
- C5 component of human complement is a plasma protein consisting of two polypeptide chains, alpha, beta, bound by disulphide bonds. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack complex.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5
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Gen-ID
- 727
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NCBI Accession
- NP_001726
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UniProt
- P01031
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Pathways
- Komplementsystem, Carbohydrate Homeostasis
Target
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