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C5 Antikörper (FITC)

Der Ziege Polyklonal Anti-C5-Antikörper wurde für IHC (fro), IP, EIA, ID und IF validiert. Er ist geeignet, C5 in Proben von Human zu detektieren.
Produktnummer ABIN568577

Kurzübersicht für C5 Antikörper (FITC) (ABIN568577)

Target

Alle C5 Antikörper anzeigen
C5 (Complement Component 5 (C5))

Reaktivität

  • 126
  • 35
  • 23
  • 16
  • 2
  • 2
  • 2
Human

Wirt

  • 118
  • 22
  • 3
  • 3
Ziege

Klonalität

  • 125
  • 21
Polyklonal

Konjugat

  • 61
  • 13
  • 11
  • 8
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser C5 Antikörper ist konjugiert mit FITC

Applikation

  • 81
  • 50
  • 44
  • 39
  • 38
  • 18
  • 13
  • 13
  • 13
  • 9
  • 6
  • 5
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF)
  • Spezifität

    The reactivity of the antiserum is restricted to C5. In immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum. The antiserum does not cross react with any other component of human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

    Produktmerkmale

    Absorption / Emission: 492 nm / 515 nm
    Molar Ratio: Fluorescein/IgG ~1.4

    Aufreinigung

    The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.

    Immunogen

    C5 protein is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization

    Isotyp

    IgG
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Rekonstitution

    Restore by adding 1 mL of sterile distilled water.

    Konzentration

    10 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives.

    Konservierungsmittel

    Without preservative

    Handhabung

    Avoid Repeated thawing and freezing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    C5 (Complement Component 5 (C5))

    Andere Bezeichnung

    Complement C5

    Hintergrund

    C5 component of human complement is a plasma protein consisting of two polypeptide chains, alpha, beta, bound by disulphide bonds. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack complex.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5

    Gen-ID

    727

    NCBI Accession

    NP_001726

    UniProt

    P01031

    Pathways

    Komplementsystem, Carbohydrate Homeostasis
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