Tested in Immunoelectrophoresis and Double Radial Immunodiffusion against normal Human serum, a single precipitin line is obtained which gives a reaction of full identity with the isolated immunogen. The product does not react with any other proteins component of Human serum or plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and double Radial Immunodiffusion.
Immunogen
The subunit C1q is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization.
C1QA
Reaktivität: Human
WB
Wirt: Maus
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore by adding 1 mL of sterile distilled water.
Konzentration
10.8 mg/mL
Buffer
PBS, pH 7.2 without preservatives.
Konservierungsmittel
Without preservative
Handhabung
Avoid Repeated thawing and freezing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
C1QA
(Complement Component 1, Q Subcomponent, A Chain (C1QA))
Andere Bezeichnung
C1q A Subunit
Hintergrund
C1 component of human complement is a complex of three glycoproteins: C1q, C1r and C1s. C1q has a molecular weight of 492.000 and is present in plasma in an average concentration of 80 mg/mL. It consists of a complex of 18 polypeptide chains of three different types, A, B and C, 6 of each type. The subcomponent C1q is responsible for the binding of C1q to immuno-complexes an membranes via the Fc portion of the immunoglobulin.Synonyms: Complement 1q, Complement C1q