Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogen
L-Lactic dehydrogenase, LDH-5(M4) isoenzyme is isolated and purified from Human placenta. Freund’s complete adjuvant is used in the first step of the immunization procedure.
LDH5
Reaktivität: Human
EIA
Wirt: Schaf
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore by adding 1.0 mL sterile distilled water.
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.Synonyms: L-Lactic dehydrogenase LDH-5 Isozyme M4