Glucuronidase beta Antikörper (Biotin)

Produktnummer ABIN568042

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Glucuronidase beta in WB, IP, IF, ID, EIA, RIA und DB. Er zeigt eine Reaktivität gegenüber E. coli.

Kurzübersicht für Glucuronidase beta Antikörper (Biotin) (ABIN568042)

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucuronidase beta Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)

Produktdetails anti-Glucuronidase beta Antikörper (Biotin)

Spezifität: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Molar Ratio: Biotin/IgG~ 6.2

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore by adding 1.0 mL of sterile distilled water.

Konzentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Details zu Glucuronidase beta

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Andere Bezeichnung: beta-Glucuronidase

Hintergrund: Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.Synonyms: Beta-G1, GUSB

Gen-ID: 946149

NCBI Accession: AP_002238

UniProt: P05804

Pathways: Glycosaminoglycan Metabolic Process