Glucuronidase beta Antikörper

Produktnummer ABIN568041

Dieser Anti-Glucuronidase beta-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von Glucuronidase beta in WB, IP, IF, ID, EIA, RIA und DB. Geeignet für E. coli.

Kurzübersicht für Glucuronidase beta Antikörper (ABIN568041)

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucuronidase beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)

Produktdetails anti-Glucuronidase beta Antikörper

Spezifität: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 1.0 mL of sterile distilled water.

Konzentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Glucuronidase beta

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Andere Bezeichnung: beta-Glucuronidase

Hintergrund: Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.Synonyms: Beta-G1, GUSB

Gen-ID: 946149

NCBI Accession: AP_002238

UniProt: P05804

Pathways: Glycosaminoglycan Metabolic Process