Collagen Type I Antikörper

Produktnummer ABIN5680217

Dieses Anti-Collagen Type I-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Collagen Type I in WB, ELISA, IF, IHC (p) und RIA. Geeignet für Human und Maus.

Kurzübersicht für Collagen Type I Antikörper (ABIN5680217)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Produktdetails anti-Collagen Type I Antikörper

Spezifität: Mouse collagen type I 100 % , mouse collagen type II and IV <0.1 %, mouse collage type III <1 %, Chicken and rat collagen type I < 0.1 % (RIA at 1:200 dilution)

Kreuzreaktivität (Details): Mouse, human, chicken and rat collagen type I < 0.1 % (RIA at 1:200 dilution)

Produktmerkmale: Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Mouse collagen type I 100 % , mouse collagen type II and IV <0.1 %, mouse collage type III <1 %, .

Aufreinigung: Affinity purified

Immunogen: Purified collagen type I from murine skin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Details zu Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Andere Bezeichnung: Collagen Type I

UniProt: P11087