Rekombinanter His Tag Antikörper

Produktnummer ABIN5668257

Der Human Chimeric Anti-His Tag-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, His Tag in Proben von zu detektieren.

Kurzübersicht für Rekombinanter His Tag Antikörper (ABIN5668257)

Target: His Tag

Antikörpertyp: Recombinant Antibody

Reaktivität: Bitte anfragen

Wirt: Human

Klonalität: Chimeric

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Klon: 3D5

Produktdetails anti-His Tag Antikörper

Verwendungszweck: Anti-His tag (C-term) [3D5], Human IgG2, kappa

Spezifität: This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: Purified his tagged Fab fragment.

Isotyp: IgG2 kappa

Anwendungsinformationen

Applikationshinweise: This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997, PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015, PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019, PMID: 30885301).

Kommentare:

This is a chimeric human antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu His Tag

Target: His Tag

Substanzklasse: Tag

Hintergrund: Polyhistidine tag