Rekombinanter EPH Receptor A2 Antikörper (AA 183-510)

Produktnummer ABIN5668240

Dieses Anti-EPH Receptor A2-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von EPH Receptor A2 in WB, ELISA, FACS, IF, Agon und INA. Geeignet für Human.

Kurzübersicht für Rekombinanter EPH Receptor A2 Antikörper (AA 183-510) (ABIN5668240)

Target: EPH Receptor A2 (EPHA2)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser EPH Receptor A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Agonist (Agon), Internalization Assay (INA)

Klon: 1C1

Produktdetails anti-EPH Receptor A2 Antikörper

Bindungsspezifität: AA 183-510

Verwendungszweck: Anti-Human Ephrin Type A receptor 2 [1C1], Rabbit IgG, kappa

Spezifität: 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM, Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, and EphA4, or any EphB receptor family members. It is reported to cross react with mouse EphA2.

Kreuzreaktivität: Maus

Produktmerkmale:

Original Species of Ab: Human

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds to human Ephrin type-A receptor 2, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Andere Bezeichnung: Ephrin Type A receptor 2

Hintergrund: EphA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK

UniProt: P29317

Pathways: RTK Signalweg