Rekombinanter F4/80 Antikörper

Produktnummer ABIN5668202

Produktdetails anti-F4/80 Antikörper

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser F4/80 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Verwendungszweck: Anti-F4/80 [Cl:A3-1 (recombinant version)], Rat IgG2b, kappa

Spezifität: This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein, expressed by murine macrophages.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: Thioglycollate stimulated peritoneal macrophages of mouse origin.

Klon: Cl:A3-1

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The anti-F4/80 antibody, clone Cl:A3-1, has been very widely used in flow cytometry, microscopy and Western blotting since its generation by Austyn and Gordon in 1980. We are the only supplier of recombinant versions of Cl:A3-1 which, whilst having the same variable domains as the original, has been shown to block hybridoma derived anti-F4/80:APC binding to in vitro generated bone marrow-derived (BMDMs) and ex vivo splenic murine macrophages. This was tested using both the recombinant version of the original isotype (rat IgG2b) as well as a murinised version (IgG2a, Ab00106-2.0). Further testing on BMDMs shows that our Fc Silent™ rat IgG2b is a valuable new reagent which shows distinct labelling of F4/80-positive cell populations, whilst the isotype control shows no binding, suggesting that only the antigen binding domains are involved in binding, promoting precision of labelling. The murinised versions will be valuable tools in the study of the role of the murine macrophage in vivo without the artefacts rat-derived antigen being co-administered may harbour. Particularly the murine IgG2a (Ab00106-2.0) should be an excellent candidate for macrophage depletion studies, with the Fc Silent™ (Ab00106-2.3) version being the ideal negative control. Using our recombinant production platform, any species and isotype are possible and our Support Team (support@absoluteantibody.com) will be glad to help you with finding the right variant to match your experimental needs.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Andere Bezeichnung: F4/80 (EMR1 Produkte)

Hintergrund: Mouse macrophage marker, EMR1 receptor, EGF-like module, Cell surface glycoprotein F4/80, EGF-like module receptor 1, Em Gpf4, TM7, Ly-71, f480, f-480, f4 80, f 480, f-4/80, f4-80

UniProt: Q61549