Separase Antikörper (AA 586-701)

Produktnummer ABIN564183

Dieses Anti-Separase-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Separase in ELISA, WB und IHC (p). Geeignet für Human. Dieses Primary Antibody wurde in 7+ Publikationen zitiert.

Kurzübersicht für Separase Antikörper (AA 586-701) (ABIN564183)

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Separase Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 6H6

Produktdetails anti-Separase Antikörper

Bindungsspezifität: AA 586-701

Verwendungszweck: Mouse monoclonal antibody raised against a partial recombinant ESPL1.

Sequenz: REELQAYKAV RADTGQERFN IICDLLELSP EETPAGAWAR ATHLVELAQV LCYHDFTQQT NCSALDAIRE ALQLLDSVRP EAQARDQLLD DKAQALLWLY ICTLEAKIQE GIERDR

Kreuzreaktivität: Human

Produktmerkmale: Antibody Reactive Against Recombinant Protein.

Immunogen: ESPL1 (NP_036423, 586 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1x PBS, pH 7.4

Handhabung: Aliquot to avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Referenzen für anti-Separase Antikörper (ABIN564183)

Mukherjee, Ge, Zhang, Edwards, Sumazin, Sharan, Rao, Medina, Pati: "MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ER?)-positive mammary adenocarcinomas." in: Oncogene, Vol. 33, Issue 48, pp. 5511-22, (2014) (PubMed).

Mukherjee, Byrd, Brawley, Bielamowicz, Li, Merchant, Maitra, Sumazin, Fuller, Kew, Sun, Powell, Ahmed, Zhang, Pati: "Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme." in: Journal of neuro-oncology, Vol. 119, Issue 1, pp. 27-35, (2014) (PubMed).

Zhang, Scorsone, Ge, Kaffes, Dobrolecki, Mukherjee, Lewis, Berg, Stephan, Pati: "Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy." in: Journal of biomolecular screening, Vol. 19, Issue 6, pp. 878-889, (2014) (PubMed).

Mukherjee, Ge, Zhang, Huang, Nakamura, Minor, Fofanov, Rao, Herron, Pati: "Separase loss of function cooperates with the loss of p53 in the initiation and progression of T- and B-cell lymphoma, leukemia and aneuploidy in mice." in: PLoS ONE, Vol. 6, Issue 7, pp. e22167, (2011) (PubMed).

Panigrahi, Zhang, Mao, Pati: "Calpain-1 cleaves Rad21 to promote sister chromatid separation." in: Molecular and cellular biology, Vol. 31, Issue 21, pp. 4335-47, (2011) (PubMed).

Meyer, Fofanov, Panigrahi, Merchant, Zhang, Pati: "Overexpression and mislocalization of the chromosomal segregation protein separase in multiple human cancers." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 8, pp. 2703-10, (2009) (PubMed).

Zhang, Ge, Meyer, Sethi, Basu, Pradhan, Zhao, Li, Cai, El-Naggar, Baladandayuthapani, Kittrell, Rao, Medina, Pati: "Overexpression of Separase induces aneuploidy and mammary tumorigenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 35, pp. 13033-8, (2008) (PubMed).

Details zu Separase

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Andere Bezeichnung: ESPL1

Hintergrund: Full Gene Name: extra spindle pole bodies homolog 1 (S. cerevisiae)
Synonyms: ESP1,FLJ46492,KIAA0165,SEPARASE,SEPARIN

Gen-ID: 9700

NCBI Accession: NM_012291