Separase Antikörper (AA 767-816)
Kurzübersicht für Separase Antikörper (AA 767-816) (ABIN1532260)
Target
Alle Separase (ESPL1) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 767-816
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Verwendungszweck
- Separase Antibody
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Spezifität
- Separase Polyclonal Antibody detects endogenous levels of Separase protein.
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Aufreinigung
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reinheit
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human SEPARASE. AA range:767-816
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Isotyp
- IgG
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Applikationshinweise
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Store at -20°C/1 year
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Haltbarkeit
- 12 months
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- Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))
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Andere Bezeichnung
- SEPARASE
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Hintergrund
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Synonyms: ESPL1, ESP1, KIAA0165, Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase
Background: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21, MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010],
Gene Name: ESPL1
Protein Name: Separin
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Molekulargewicht
- 233 kDa
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Gen-ID
- 9700
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OMIM
- 604143
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UniProt
- Q14674
Target
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