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MUTYH Antikörper (AA 436-535)

Name: MUTYH Antikörper (AA 436-535)
SKU: ABIN561883
Price: 693.44 EUR
Availability: InStock

MUTYH Reaktivität: Human WB, ELISA, IP, RNAi Wirt: Maus Monoclonal 4D10 unconjugated

MUTYH monoclonal antibody (M01), clone 4D10 Western Blot analysis of MUTYH expression in Hela S3 NE . (MUTYH Antikörper (AA 436-535))

Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). (MUTYH Antikörper (AA 436-535))

Detection limit for recombinant GST tagged MUTYH is approximately 1ng/ml as a capture antibody. (MUTYH Antikörper (AA 436-535))

ELISA

Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10. (MUTYH Antikörper (AA 436-535))

Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody. (MUTYH Antikörper (AA 436-535))

Western Blot detection against Immunogen (36. (MUTYH Antikörper (AA 436-535))

6 Abbildungen

(7 Referenzen)

Produktnummer ABIN561883

Datenblatt (PDF)

Target Alle MUTYH Antikörper anzeigen

MUTYH (MutY Homolog (E. Coli) (MUTYH))
Bindungsspezifität
16

5

5

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 436-535
Reaktivität
51

32

13

4

2

2

2

2

2

2

2

2

2

2

2

2

1
Human
Wirt
45

3

3
Maus
Passende Sekundärantikörper
Klonalität
48

3
Monoklonal
Konjugat
31

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser MUTYH Antikörper ist unkonjugiert
Applikation
28

17

16

13

13

12

10

7

4

3

2

2

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), RNA Interference (RNAi)

Verwendungszweck

Mouse monoclonal antibody raised against a partial recombinant MUTYH.

Sequenz

KLTYQVYGLA LEGQTPVTTV PPGARWLTQE EFHTAAVSTA MKKVFRVYQG QQPGTCMGSK RSQVSSPCSR KKPRMGQQVL DNFFRSHIST DAHSLNSAAQ

Kreuzreaktivität

Human

Produktmerkmale

Antibody Reactive Against Recombinant Protein.

Immunogen

MUTYH (AAH03178, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Klon

4D10

Isotyp

IgG1
anti-MutY Homolog (E. Coli) (MUTYH) (pan) antibody
MUTYH Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 341-367) antibody
MUTYH Reaktivität: Human, Maus WB, IF, FACS Wirt: Kaninchen Polyclonal RB17460 unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Internal Region) antibody
MUTYH Reaktivität: Human, Maus, Ratte WB, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 1-265) antibody
MUTYH Reaktivität: Human WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (C-Term) antibody
MUTYH Reaktivität: Human, Maus, Ratte, Affe, Ziege, Schaf, Kaninchen, Schwein, Pferd, Hamster, Meerschweinchen, Esel, Hund, Rind (Kuh), Huhn, Cat, Avian WB Wirt: Ziege Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 436-535) antibody
MUTYH Reaktivität: Human WB, ELISA, IF Wirt: Maus Monoclonal 1C8 unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Center) antibody
MUTYH Reaktivität: Human, Maus WB Wirt: Kaninchen Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) antibody
MUTYH Reaktivität: Human, Maus, Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Center) antibody
MUTYH Reaktivität: Human, Maus, Ratte, Affe WB, IHC, IF, IC Wirt: Kaninchen Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 352-381), (Middle Region) antibody
MUTYH Reaktivität: Human, Maus WB, IF, FACS, EIA Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Optimal working dilution should be determined by the investigator.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Buffer

In 1x PBS, pH 7.4

Handhabung

Aliquot to avoid repeated freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Komine, Shimodaira, Takao, Soeda, Zhang, Takahashi, Ishioka: "Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia Coli Strain." in: Human mutation, Vol. 36, Issue 7, pp. 704-11, (2015) (PubMed).

Shinmura, Goto, Tao, Kato, Suzuki, Nakamura, Matsuda, Yin, Morita, Kono, Sugimura: "Impaired 8-hydroxyguanine repair activity of MUTYH variant p.Arg109Trp found in a Japanese patient with early-onset colorectal cancer." in: Oxidative medicine and cellular longevity, Vol. 2014, pp. 617351, (2014) (PubMed).

Agustina, Hahm, Han, Tran, Chung, Park, Park, Han: "Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation." in: BMC molecular biology, Vol. 15, pp. 17, (2014) (PubMed).

Raetz, Xie, Kundu, Brinkmeyer, Chang, David: "Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells." in: Carcinogenesis, Vol. 33, Issue 11, pp. 2301-9, (2012) (PubMed).

Hahm, Chung, Agustina, Han, Yoon, Park, Kang, Park, Na, Han: "Human MutY homolog induces apoptosis in etoposide-treated HEK293 cells." in: Oncology letters, Vol. 4, Issue 6, pp. 1203-1208, (2012) (PubMed).

Suzuki, Harashima, Kamiya: "Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine." in: DNA repair, Vol. 9, Issue 5, pp. 542-50, (2010) (PubMed).

van der Post, Kets, Ligtenberg, van Krieken, Hoogerbrugge: "Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis." in: Virchows Archiv : an international journal of pathology, Vol. 454, Issue 1, pp. 25-9, (2008) (PubMed).
Target

MUTYH (MutY Homolog (E. Coli) (MUTYH))

Andere Bezeichnung

MUTYH (MUTYH Produkte)

Hintergrund

Full Gene Name: mutY homolog (E. coli)
Synonyms: MGC4416,MYH

Gen-ID

4595

Pathways

DNA Reparatur