beta Actin Antikörper (N-Term)

Produktnummer ABIN5611427

Der Kaninchen Polyklonal Anti-beta Actin-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, beta Actin in Proben von Human, Maus, Ratte, Huhn, Kaninchen, Hund, Schwein, Rind (Kuh), Schaf, Meerschweinchen und Cat zu detektieren.

Kurzübersicht für beta Actin Antikörper (N-Term) (ABIN5611427)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Maus, Ratte, Huhn, Kaninchen, Hund, Schwein, Rind (Kuh), Schaf, Meerschweinchen, Cat

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-beta Actin Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody recognizes Actin-Beta (ACTB).

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Cat, Chicken, Canine (Dog), Guinea Pig, Mouse, Porcine, Rabbit, Rat and Sheep.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: N-terminal Human beta Actin.

Anwendungsinformationen

Applikationshinweise: Western blot. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by Alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS, pH 7.4 containing BSA and Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: Actin beta / ACTB

Hintergrund: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three alpha-actins (skeletal, cardiac, and smooth muscle), one beta actin (non-muscle), and two gamma-actins (gamma-smooth muscle and gamma-nonmuscle). Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.Synonyms: Actin cytoplasmic 1, Beta-Actin

Gen-ID: 60

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction