GCLC Antikörper (AA 528-637)

Produktnummer ABIN561050

Der Maus Monoklonal Anti-GCLC-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, GCLC in Proben von Human zu detektieren. Es sind 6+ Publikationen verfügbar.

Kurzübersicht für GCLC Antikörper (AA 528-637) (ABIN561050)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GCLC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Klon: 3H1

Produktdetails anti-GCLC Antikörper

Bindungsspezifität: AA 528-637

Verwendungszweck: Mouse monoclonal antibody raised against a partial recombinant GCLC.

Sequenz: EGVFPGLIPI LNSYLENMEV DVDTRCSILN YLKLIKKRAS GELMTVARWM REFIANHPDY KQDSVITDEM NYSLILKCNQ IANELCECPE LLGSAFRKVK YSGSKTDSSN

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Antibody Reactive Against Recombinant Protein.

Immunogen: GCLC (NP_001489, 528 a.a. ~ 637 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1x PBS, pH 7.4

Handhabung: Aliquot to avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Referenzen für anti-GCLC Antikörper (ABIN561050)

Sugiyama, Ebinuma, Nakamoto, Sakasegawa, Murakami, Chu, Usui, Ishibashi, Wakayama, Taniki, Murata, Saito, Fukasawa, Saito, Yamagishi, Wakita, Takaku, Hibi, Saito, Kanai: "Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus." in: PLoS ONE, Vol. 9, Issue 4, pp. e94460, (2014) (PubMed).

Venè, Cardinali, Arena, Ferrari, Benelli, Minghelli, Poggi, Noonan, Albini, Tosetti: "Glycogen synthase kinase 3 regulates cell death and survival signaling in tumor cells under redox stress." in: Neoplasia (New York, N.Y.), Vol. 16, Issue 9, pp. 710-22, (2014) (PubMed).

Li, Gunter, Fukagawa: "Differential activation of the inflammasome in THP-1 cells exposed to chrysotile asbestos and Libby "six-mix" amphiboles and subsequent activation of BEAS-2B cells." in: Cytokine, Vol. 60, Issue 3, pp. 718-30, (2012) (PubMed).

Fan, Liu, Hao, Wang, Robinson, Monnier: "The LEGSKO mouse: a mouse model of age-related nuclear cataract based on genetic suppression of lens glutathione synthesis." in: PLoS ONE, Vol. 7, Issue 11, pp. e50832, (2012) (PubMed).

Abel, Bubel, Simper, Powell, McClellan, Andreeff, MacLeod, DiGiovanni: "Protection against 2-chloroethyl ethyl sulfide (CEES)-induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway." in: Toxicology and applied pharmacology, Vol. 255, Issue 2, pp. 176-83, (2011) (PubMed).

Hardwick, Fisher, Canet, Lake, Cherrington: "Diversity in antioxidant response enzymes in progressive stages of human nonalcoholic fatty liver disease." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 38, Issue 12, pp. 2293-301, (2010) (PubMed).

Details zu GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung: GCLC

Hintergrund: Full Gene Name: glutamate-cysteine ligase, catalytic subunit
Synonyms: GCS,GLCL,GLCLC

Gen-ID: 2729

NCBI Accession: NM_001498

Pathways: Cell RedoxHomeostasis