Clusterin Antikörper

Produktnummer ABIN5596991

Produktdetails anti-Clusterin Antikörper

Target: Clusterin (CLU)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Clusterin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Aufreinigung: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Immunogen:

Immunogen: apoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:5,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-Clusterin Antikörper (ABIN5596991)

Hayashi, Campenot, Vance, Vance: "Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin." in: The Journal of biological chemistry, Vol. 284, Issue 43, pp. 29605-13, (2009) (PubMed).

Details zu Clusterin

Target: Clusterin (CLU)

Andere Bezeichnung: Apolipoprotein J (CLU Produkte)

Synonyme: LOC100136120 antikoerper, clu antikoerper, MGC53055 antikoerper, zgc:73352 antikoerper, xclu antikoerper, MGC89163 antikoerper, CLU antikoerper, APO-J antikoerper, APOJ antikoerper, CLI antikoerper, CLU1 antikoerper, CLU2 antikoerper, KUB1 antikoerper, NA1/NA2 antikoerper, SGP-2 antikoerper, SGP2 antikoerper, SP-40 antikoerper, TRPM-2 antikoerper, TRPM2 antikoerper, DAG antikoerper, RATTRPM2B antikoerper, SP40 antikoerper, TRPM2B antikoerper, Trpm2 antikoerper, Trpmb antikoerper, AI893575 antikoerper, ApoJ antikoerper, Cli antikoerper, D14Ucla3 antikoerper, Sgp-2 antikoerper, Sgp2 antikoerper, Sugp-2 antikoerper, GP80 antikoerper, gpIII antikoerper, 40 antikoerper, clusterin-1 antikoerper, clusterin L homeolog antikoerper, clusterin antikoerper, Clusterin antikoerper, LOC100136120 antikoerper, clu.L antikoerper, clu antikoerper, CLU antikoerper, clus antikoerper, Clu antikoerper, LOC101113728 antikoerper

Hintergrund:

Synonyms: Clusterin, Complement-associated protein SP-40,40, Complement cytolysis inhibitor, CLI, NA1/NA2, Apolipoprotein J, Apo-J, Testosterone-repressed prostate message 2, TRPM-2, Ku70-binding protein 1, Aging-associated gene 4 protein

Background: This antibody is suitable for cardiovascular research. Cleaved into the following 2 chains: 1- Recommended name: Clusterin beta chain Alternative name(s): ApoJalpha Complement cytolysis inhibitor a chain 2- Recommended name: Clusterin alpha chain Alternative name(s): ApoJbeta Complement cytolysis inhibitor b chain

Gene Name: CLU, APOJ, CLI, KUB1

Gen-ID: 1191

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling