TET3 Antikörper (N-Term)

Produktnummer ABIN5596980

Produktdetails anti-TET3 Antikörper

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TET3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): It is directed against, and shows specific reactivity for Tet3 protein. Cross reactivity with Tet1 and Tet2 has not been determined.

Aufreinigung: Anti-TET3 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.

Immunogen:

Immunogen: TET3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-3 domain containing the N-terminal 160 amino acids of the protein.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: Tet3 antibody has been tested for use by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~180 kDa.

Western Blot Dilution: 1:5000

ELISA Dilution: 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 70 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu TET3

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Andere Bezeichnung: TET3 (TET3 Produkte)

Synonyme: B430006D22Rik antikoerper, BC037432 antikoerper, D230004J03Rik antikoerper, tet methylcytosine dioxygenase 3 antikoerper, Tet3 antikoerper, TET3 antikoerper

Hintergrund:

Synonyms: TET antibodies, Anti-TET Methylcytosine Dioxygenase 3, Anti-TET3 polyclonal antibody, KIAA0401 antibody, dioxygenase antibodies

Background: Anti-Tet3 is expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes. Anti-Tet3 is involved in the epigenetic chromatin reprogramming of a zygote after its fertilization, and the demethylation occurs before the first cell division in the paternal pronucleus. Tet 3 antibody cannot bind to the maternal pronucleus chromatin due to DPPA3/PGC7 chromatin presence. Tet 3 functions as the dioxygenase which synthesizes a hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet3 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet3 plays an active role in catalyzing those conversions which have been suggested as the first step for cytosine DNA de-methylation. Tet 3 also recruits O-GlcNAc transferase OGT to aid H2B GlcNAcylation by OGT. Anti-TET3 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.

Gen-ID: 200424

UniProt: O43151

Pathways: Warburg Effekt