GAPDH Antikörper

Produktnummer ABIN559691

Der Maus Monoklonal Anti-GAPDH-Antikörper wurde für WB, ELISA, DB und ISt validiert. Er ist geeignet, GAPDH in Proben von Human, Maus, Ratte, Kaninchen, Huhn, Hamster, Saccharomyces cerevisiae, Insekten und Bakterien zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für GAPDH Antikörper (ABIN559691)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Maus, Ratte, Kaninchen, Huhn, Hamster, Saccharomyces cerevisiae, Insekten, Bakterien

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB), Immunostaining (ISt)

Klon: GA1R

Produktdetails anti-GAPDH Antikörper

Spezifität: Recognizes native and denatured forms of GAPDH (∼37kDa). GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster / GAPDH from other species may also be detectable

Aufreinigung: Protein A affinity chromatography from mouse ascites fluid

Immunogen: Recombinant GAPDH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-GAPDH Antikörper (ABIN559691)

Steinbrecht, Pfeifer, Herzog, Katzenberger, Schulz, Kammerer, Küpper: "HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells." in: Toxicology letters, Vol. 319, pp. 155-159, (2019) (PubMed).

Martínez-Moya, Ortega-González, González, Anzola, Ocón, Hernández-Chirlaque, López-Posadas, Suárez, Zarzuelo, Martínez-Augustin, Sánchez de Medina: "Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats." in: Pharmacological research : the official journal of the Italian Pharmacological Society, Vol. 66, Issue 2, pp. 144-53, (2012) (PubMed).

Gandhi, Takahashi, Virtanen, Syed, Davey, Mahomed: "Microarray analysis of the infrapatellar fat pad in knee osteoarthritis: relationship with joint inflammation." in: The Journal of rheumatology, Vol. 38, Issue 9, pp. 1966-72, (2011) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH