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Phosphoserine Antikörper (HRP)

Der Kaninchen Polyklonal Anti-Phosphoserine-Antikörper wurde für WB, ELISA, IP und IHC validiert. Er ist geeignet, Phosphoserine in Proben von zu detektieren.
Rockland
Produktnummer ABIN5596875
Hersteller Produkt- Nr.: 600-403-261

Kurzübersicht für Phosphoserine Antikörper (HRP) (ABIN5596875)

Target

Phosphoserine

Reaktivität

Bitte anfragen

Wirt

  • 28
  • 11
Kaninchen

Klonalität

  • 29
  • 10
Polyklonal

Konjugat

  • 15
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Phosphoserine Antikörper ist konjugiert mit HRP

Applikation

  • 37
  • 17
  • 15
  • 11
  • 7
  • 6
  • 6
  • 5
  • 4
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
  • Hersteller Produkt- Nr.

    600-403-261

    Hersteller

    Rockland

    Verwendungszweck

    Phosphoserine Antibody Peroxidase Conjugated

    Kreuzreaktivität (Details)

    Phosphoserine antibody detects proteins phosphorylated on serine residues.

    Produktmerkmale

    Synonyms: Phosphoserine [pS] reactive antibody, Phosphoserine [pS] polyclonal antibody, Phosphoserine antibody produced in rabbit

    Aufreinigung

    Anti-Phosphoserine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: Anti-Phosphoserine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.

    Immunogen Type: Other

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: HRP Conjugated Anti-Phosphoserine antibody is suitable for use in ELISA and western blotting assays.  The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues.  The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

    Western Blot Dilution: 1:500

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:5,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 40 % (v/v) Glycerol

    Preservative: None. Do NOT add sodium azide!

    Konservierungsmittel

    Without preservative

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store pS antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    Phosphoserine

    Substanzklasse

    Amino Acid

    Hintergrund

    Background: Anti-Phosphoserine Antibody recognizes phosphoserine, peptidyl-phosphoserine, and serine-phosphorylated proteins
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