Phosphotyrosine Antikörper (Biotin)

Produktnummer ABIN5596786

Hersteller Produkt- Nr.: 600-406-262

Der Biotin-konjugierte Kaninchen Polyklonal anti-Phosphotyrosine Antikörper (ABIN5596786) detektiert spezifisch Phosphotyrosine in WB, IP, FACS, IHC und ELISA.

Kurzübersicht für Phosphotyrosine Antikörper (Biotin) (ABIN5596786)

Target: Phosphotyrosine

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Produktdetails anti-Phosphotyrosine Antikörper (Biotin)

Hersteller Produkt- Nr.: 600-406-262

Hersteller: Rockland

Verwendungszweck: Phosphotyrosine Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.

Produktmerkmale: Synonyms: PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit

Aufreinigung: Anti-PhosphoTyrosine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose followed by conjugation to biotin and subsequent purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-PhosphoTyrosine was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-PhosphoTyrosine antibody is tested for use in ELISA, immunohistochemistry-P, IP, and western blotting assays.  The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues.  The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA and flow cytometry.

Western Blot Dilution: 1:1,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Anti-pY at -20° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Background: Anti-PhosphoTyrosine detects phosphorylated Tyrosines. Tyrosine is one of the 22 amino acids that are used by cells to synthesize proteins. Tyrosine has a phenol group. It functions as a receiver of phosphate groups that are transferred by way of protein kinases. Phosphorylation of the hydroxyl group changes the activity of the target protein. Anti-PhosphoTyrosine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.