Phosphotyrosine Antikörper

Produktnummer ABIN5596785

Hersteller Produkt- Nr.: 600-401-262

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-Phosphotyrosine Antikörper wird verwendet zum Nachweis von Phosphotyrosine. Er wurde validiert für WB, IP, IHC und ELISA.

Kurzübersicht für Phosphotyrosine Antikörper (ABIN5596785)

Target: Phosphotyrosine

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA

Produktdetails anti-Phosphotyrosine Antikörper

Hersteller Produkt- Nr.: 600-401-262

Hersteller: Rockland

Verwendungszweck: Phosphotyrosine Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.

Produktmerkmale: Synonyms: PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit

Aufreinigung: Anti-PhosphoTyrosine (pY) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This Anti-PhosphoTyrosine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-PhosphoTyrosine (pY) Antibody is tested for use in ELISA, western blotting, immunohistochemistry-P, immunoprecipitation, and Non-radioactive Tyrosine kinase assays.  Anti-PhosphoTyrosine (pY) Antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. Anti-PhosphoTyrosine (pY) Antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, Anti-PhosphoTyrosine (pY) Antibody is likely functional in RIA and flow cytometry.

Western Blot Dilution: 1:250

Immunoprecipitation Dilution: 10.0 μg/ 500 μg sample

ELISA Dilution: 1:1,000

Other: Non-radioactive Tyrosine Kinase Assay: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store PhosphoTyrosine antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Phosphotyrosine Antikörper (ABIN5596785)

Koytiger, Kaushansky, Gordus, Rush, Sorger, MacBeath: "Phosphotyrosine signaling proteins that drive oncogenesis tend to be highly interconnected." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 5, pp. 1204-13, (2013) (PubMed).

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Background: Phosphotyrosine is the tyrosine amino acid that has been phosphorylated and is considered one of the integral steps in signal transduction and general enzyme activity. Tyrosine is phosphorylated through protein kinases and through phosphorylation, phosphotyrosine can bind other proteins causing binding of multi-protein complexes that connect the upstream kinases and downstream effectors. Phosphotyrosine-binding domains, or PTB's, are pervasive throughout the study of signal transduction and disease. Anti-Phosphotyrosine (pY) Antibody is ideal for investigators in Cancer, Signal Transduction, and Cell Signaling research.