PPARG Antikörper (pSer273)

Produktnummer ABIN5596784

Hersteller Produkt- Nr.: 600-401-j94

Produktdetails anti-PPARG Antikörper

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Bindungsspezifität: pSer273

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPARG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Hersteller Produkt- Nr.: 600-401-j94

Hersteller: Rockland

Verwendungszweck: PPAR gamma phospho S273 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100 % sequence homology.

Produktmerkmale: Synonyms: rabbit anti-PPAR gamma pS273 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma

Aufreinigung: Anti-PPAR gamma pS273 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human PPAR gamma at the S273 residue.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-PPAR gamma pS273 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-PPAR antibody has been tested by ELISA and dot blot and is useful for Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~57 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Andere Bezeichnung: PPARG (PPARG Produkte)

Hintergrund: Background: PPAR has a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. The receptor, when activated by ligand, binds to a promotor element in the gene for acyl-CoA oxidase and activates its transcription. In this way it controls the peroxisomal beta-oxidation pathway of fatty acids. It plays a key role in regulating adipocyte differentiation, gut homeostasis, and glucose homeostasis. Anti-PPAR gamma pS273 Antibody is ideal for researchers interested in Metabolism, Cardiovascular or Neuroscience research.

Gen-ID: 5468, 20336229

UniProt: P37231

Pathways: MAPK Signalweg, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation