DNASE1 Antikörper (HRP)

Produktnummer ABIN5596707

Produktdetails anti-DNASE1 Antikörper (HRP)

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNASE1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Deoxyribonuclease I from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas].

Immunogen:

Immunogen: Deoxyribonuclease I [Bovine Pancreas]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Deoxyribonuclease I has been assayed against 1.0 μg of Deoxyribonuclease I [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu DNASE1

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Andere Bezeichnung: Deoxyribonuclease I (DNASE1 Produkte)

Synonyme: LOC397802 antikoerper, DNL1 antikoerper, DRNI antikoerper, AI788650 antikoerper, DNaseI antikoerper, Dnl1 antikoerper, dnase1-A antikoerper, zgc:92440 antikoerper, DNase I antikoerper, deoxyribonuclease 1 antikoerper, deoxyribonuclease I antikoerper, deoxyribonuclease I L homeolog antikoerper, Deoxyribonuclease I antikoerper, endA antikoerper, LOC397802 antikoerper, DNASE1 antikoerper, Dnase1 antikoerper, dnase1.L antikoerper, dnase1 antikoerper, CpipJ_CPIJ011184 antikoerper, CpipJ_CPIJ011185 antikoerper, CpipJ_CPIJ011186 antikoerper, CpipJ_CPIJ011187 antikoerper, CpipJ_CPIJ011190 antikoerper, CpipJ_CPIJ017803 antikoerper, Desal_1409 antikoerper, MPET_RS05255 antikoerper, METEV_RS02275 antikoerper, Plabr_0631 antikoerper

Hintergrund:

Synonyms: Deoxyribonuclease-1 EC=3.1.21.1 Deoxyribonuclease I DNase I

Background: Deoxyribonuclease I (DNase I) is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.

Gene Name: DNASE1, DNL1

Gen-ID: 282217

UniProt: P00639