CRISPR-Cas9 (C-Term) Antikörper

Produktnummer ABIN5596701

Hersteller Produkt- Nr.: 600-401-gk0

This anti- antibody (ABIN5596701) is a Rabbit Polyclonal antibody detecting in WB, IF, ELISA, FM, MA. Suitable for Streptococcus pyogenes.

Kurzübersicht für CRISPR-Cas9 (C-Term) Antikörper (ABIN5596701)

Target: CRISPR-Cas9

Reaktivität: Streptococcus pyogenes

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Fluorescence Microscopy (FM), Multiplex Assay (MA)

Produktdetails

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-gk0

Hersteller: Rockland

Verwendungszweck: Cas 9 Antibody

Kreuzreaktivität (Details): Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.

Produktmerkmale: Synonyms: rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA, Immunofluorescence, and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:25,000-1:30,000

IF Microscopy Dilution: 1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: CRISPR-Cas9

Andere Bezeichnung: cas9

Hintergrund: Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

Gen-ID: 901176

NCBI Accession: NP_269215

UniProt: Q99ZW2