Rekombinanter TET1 Antikörper (AA 1682-1914) (Sortase,His tag,AVI tag)

Produktnummer ABIN5563986

Der Maus Monoklonal anti-TET1 Antikörper wird verwendet zum Nachweis von TET1 in Proben von Human und Maus. Er wurde validiert für ChIP-seq.

Kurzübersicht für Rekombinanter TET1 Antikörper (AA 1682-1914) (Sortase,His tag,AVI tag) (ABIN5563986)

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TET1 Antikörper ist konjugiert mit Sortase,His tag,AVI tag

Applikation: ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-TET1 Antikörper (Sortase,His tag,AVI tag)

Bindungsspezifität: AA 1682-1914

Verwendungszweck: AbFlex TET1 antibody (rAb)

Marke: AbFlex®

Produktmerkmale: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against a recombinant protein comprising amino acids 1682-1914 of mouse TET1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 4 µL per ChIP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61741.

Kommentare:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu TET1

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Andere Bezeichnung: TET1

Hintergrund: AbFlex TET1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TET1(Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via TET proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. TET1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters.

Molekulargewicht: 215 kDa

NCBI Accession: NP_001240786

Pathways: Stem Cell Maintenance, Warburg Effekt