YY1 Antikörper (AA 249-414)

Produktnummer ABIN5563972

Der Kaninchen Polyklonal anti-YY1 Antikörper (ABIN5563972) detektiert spezifisch YY1 in WB, IHC, IP, ChIP-seq und CUT&Tag. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für YY1 Antikörper (AA 249-414) (ABIN5563972)

Target: YY1 (YY1 Transcription Factor (YY1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser YY1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Produktdetails anti-YY1 Antikörper

Bindungsspezifität: AA 249-414

Verwendungszweck: YY1 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a recombinant protein corresponding to amino acids 249-414 of human YY1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 10 µL per ChIP IP: 0.5 - 4 µL per IP WB*: 1:1,000 - 1:5,000 dilution IHC (FFPE): 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Andere Bezeichnung: YY1

Hintergrund: YY1 (Yin Yang 1) is a transcription factor that belongs to the GLI-Kruppel class of zinc finger transcription factors and is ubiquitously expressed. YY1 is known to have a fundamental role in normal biologic processes such as embryogenesis, differentiation, replication and cellular proliferation. YY1 exerts its effects on genes involved in these processes via its ability to initiate, activate or repress transcription depending upon the context in which it binds. Since expression and function of YY1 are known to be intimately associated with progression through phases of the cell cycle, the physiologic significance of YY1 activity has been applied to models of tumor biology. It can repress and activate a number of gene promoters and direct histone deacetylases (HDACs) and histone acetyltransferases (HATs).

Molekulargewicht: 63 kDa

NCBI Accession: NP_003394