YY1 Antikörper

Produktnummer ABIN2443872

Zitiert in 1 Publikation. Der Huhn Polyklonal anti-YY1 Antikörper (ABIN2443872) detektiert spezifisch YY1 in WB und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Schaf und Schwein.

Kurzübersicht für YY1 Antikörper (ABIN2443872)

Target: YY1 (YY1 Transcription Factor (YY1))

Reaktivität: Human, Maus, Ratte, Schaf, Schwein

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser YY1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-YY1 Antikörper

Verwendungszweck: YY1 Chicken Polyclonal Antibody

Spezifität: The antibody recognizes the Transcription Factor YY1, in WB, the antibody recognizes the O-glycosylated, the phosphorylated, and the post-translationally unmodified protein in cellular and tissue lysates.

Aufreinigung: Purified

Immunogen: Recombinant human YY1

Anwendungsinformationen

Applikationshinweise: WB 1:3000,IF 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 30 μL: WB 1:3000 - 1:8000, IF 1:500 - 1:1000 (in tissue paraffin sections). Optimal dilution has to be determined by the user.

Buffer: Lyophilized purified total IgY in PBS pH 7.4

Konservierungsmittel: Sodium azide, Streptomycin

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Referenzen für anti-YY1 Antikörper (ABIN2443872)

Gelev, Zabolotny, Lange, Hiromura, Yoo, Orlando, Kushnir, Horikoshi, Paquet, Bachvarov, Schaffer, Usheva: "A new paradigm for transcription factor TFIIB functionality." in: Scientific reports, Vol. 4, pp. 3664, (2015) (PubMed).

Details zu YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Andere Bezeichnung: YY1

Hintergrund: YY1 is a ubiquitous and multifunctional zinc-finger transcription factor involved in the activation or repression of a large number of genes that are critical for basic biological processes of cell growth, development, differentiation, cell cycle and even programmed cell death (apoptosis). YY1 is an essential protein, deletion of YY1 leads to lethality in mouse embryos and disruption of one allele causing developmental defects.

UniProt: P25490