CRISPR-Cas9 Antikörper

Produktnummer ABIN5563966

Dieser Anti-CRISPR-Cas9-Antikörper ist ein Maus-Monoklonal-Antikörper zum Nachweis von CRISPR-Cas9 in WB. Geeignet für Streptococcus pyogenes.

Kurzübersicht für CRISPR-Cas9 Antikörper (ABIN5563966)

Target: CRISPR-Cas9

Reaktivität: Streptococcus pyogenes

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Klon: 11E7-

Produktdetails

Aufreinigung: Affinity Purified

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Validated Applications: WB: 0.5 - 2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Antigendetails

Target: CRISPR-Cas9

Andere Bezeichnung: Cas9

Hintergrund: SaCas9 is a nuclease from Staphylococcus aureus that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Molekulargewicht: 130 kDa