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Rekombinanter Rpb1 CTD Antikörper (pSer5)

Reaktivität: Human WB, IF, ICC, ChIP, ChIP-seq, ELISA Wirt: Maus Monoclonal unconjugated Recombinant Antibody
Produktnummer ABIN5563956
  • Target Alle Rpb1 CTD Produkte
    Rpb1 CTD (RNA Polymerase II CTD)
    Antikörpertyp
    Recombinant Antibody
    Bindungsspezifität
    • 14
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    pSer5
    Reaktivität
    • 37
    • 21
    • 16
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 22
    • 11
    • 4
    Maus
    Klonalität
    • 22
    • 15
    Monoklonal
    Konjugat
    • 23
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser Rpb1 CTD Antikörper ist unkonjugiert
    Applikation
    • 23
    • 16
    • 13
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), ELISA
    Marke
    AbFlex®
    Spezifität
    Human
    Produktmerkmale
    AbFlex™ RNA pol II CTD phospho Ser5 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol IIcontains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.
    Aufreinigung
    Ni-NTA
    Isotyp
    IgG2a
  • Applikationshinweise
    ChIP-Seq: 4 μg each ICC/IF: 1 - 2 μg / mL WB: 0.5 - 2 μg/mL Bead-based ELISA: 0.1 - 5 μg/mL
    Kommentare

    AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
    AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

    AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
  • Target
    Rpb1 CTD (RNA Polymerase II CTD)
    Andere Bezeichnung
    RNA pol II CTD (Rpb1 CTD Produkte)
    Synonyme
    5 antikoerper, 8WG16 antikoerper, CG1554 antikoerper, CTD antikoerper, Dmel\\CG1554 antikoerper, H5 antikoerper, II antikoerper, L5 antikoerper, POL antikoerper, Pol II antikoerper, Pol II CTD antikoerper, Pol II Ser5P antikoerper, Pol II0[ser2] antikoerper, Pol II0[ser5] antikoerper, Pol II[ser2] antikoerper, Pol IIa antikoerper, Pol IIo antikoerper, Pol IIo[Ser2] antikoerper, Pol IIo[ser2] antikoerper, Pol IIo[ser5] antikoerper, Pol-IIa antikoerper, PolII antikoerper, PolIIo antikoerper, RNA Pol II antikoerper, RNA Pol II CTD antikoerper, RNA PolI 215 antikoerper, RNA PolII antikoerper, RNA pol II antikoerper, RNA pol IIo antikoerper, RNA polII antikoerper, RNAP antikoerper, RNAP II antikoerper, RNAP II LS antikoerper, RNAPII antikoerper, RNAPII0 antikoerper, RNApol2 antikoerper, RNApolII antikoerper, RPB1 antikoerper, RPII215 antikoerper, RpII antikoerper, Rpb1 antikoerper, Rpll215 antikoerper, Ubl antikoerper, dRPB1 antikoerper, dRpb1 antikoerper, l(1)10Ca antikoerper, l(1)DC912 antikoerper, l(1)DF912 antikoerper, l(1)G0040 antikoerper, l(1)L5 antikoerper, pol II antikoerper, polII antikoerper, RNA polymerase II 215kD subunit antikoerper, RpII215 antikoerper
    Molekulargewicht
    240 kDa
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