SYN1 Antikörper (AA 362-511)

Produktnummer ABIN5542390

Dieses Anti-SYN1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SYN1 in WB, ELISA, IHC, ICC und FACS. Geeignet für Human, Ratte, Maus und Affe. Unabhängig validiert für die Verwendung in Multiplex Immunohistochemistry.

Kurzübersicht für SYN1 Antikörper (AA 362-511) (ABIN5542390)

Target: SYN1 (Synapsin I (SYN1))

Reaktivität: Human, Ratte, Maus, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SYN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 7H10G6

Produktdetails anti-SYN1 Antikörper

Bindungsspezifität: AA 362-511

Verwendungszweck: SYN1 Antibody

Aufreinigung: Purified antibody

Immunogen: Purified recombinant fragment of human SYN1 (AA: 362-511) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (mIHC)

Validierung #104335 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104335

Datum: 20.04.2021

Antigen: SYN1

Chargennummer: 151229

Validierte Anwendung: Multiplex Immunohistochemistry

Positivkontrolle:

FFPE normal human cortex

Negativkontrolle:

Unlabeled control (FFPE normal human cortex)

Bewertung:

Passed. The anti-SYN1 antibody ABIN5542390 produces punctate labeling in synapse-rich regions of the human cortex. This pattern is consistent with expected SYN1 localization in synaptic contacts.

Validierungsbilder

FFPE normal human cortex tissue section labeled with anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SLC32A1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 500) and anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488).

FFPE normal human cortex tissue section labeled with anti-SYN1 antibody ABIN5542390 (red; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and DLG4 were labeled with anti-MAP2 antibody ABIN125739 (cyan; bound to fluorophore ATTO 550) and anti-DLG4 antibody ABIN361694 (green; bound to fluorophore ATTO 550).

Protokoll

Primärantikörper: ABIN5542390

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SYN1 antibody ABIN5542390 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Anmerkungen:

• No signal was detected in unlabeled specimens.

• Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handhabung

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Details zu SYN1

Target: SYN1 (Synapsin I (SYN1))

Andere Bezeichnung: SYN1

Hintergrund: This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molekulargewicht: 74.1 kDa

Gen-ID: 6853

UniProt: P17600