MASP1 Antikörper

Produktnummer ABIN5542172

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch MASP1 in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MASP1 Antikörper (ABIN5542172)

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser MASP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 38:12-3b

Produktdetails anti-MASP1 Antikörper

Spezifität: The monoclonal antibody 38:12-3 recognizes MASP-3, a 94 kDa mannan-binding lectin (MBL)-associated serine protease. The monoclonal antibody 38:12-3 does not recognize MASP-1.

Aufreinigung: Purified

Immunogen: Purified MASP-3

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immuno Assays (Detector). Western blot. The typical starting working dilution is 1/50.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.02 % Sodium Azide Stabilizers: 0.1 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu MASP1

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Andere Bezeichnung: masp-1

Hintergrund: Three pathways of complement activation have been reported: the antibody-dependent classical pathway, the antibody-independent alternative pathway and the lectin pathway. Activation of each pathway involves formation of serine protease complexes, which results in activation of the central complement component, C3. In the lectin pathway, mannose- binding-lectin (MBL)-associated serine proteases (MASP) form complexes with oligopolymeric lectin molecules, which are involved in pattern recognition. Upon binding of the recognition molecules to carbohydrates on the surface of microorganisms, MASPs are converted to their active forms and initiate complement activation. Six members of the family of MASP-like serine proteases have been reported: MASP-1, MASP-2, MASP-3, Map19, C1r and C1s. These products show identical domain organization, even though the overall amino acid sequence identity is only approximately 40 % . Each MASP-type forms homodimers and is individually associated with MBL and the ficolins in a Ca 2+ -dependent matter. The four MBL-associated proteins are generated from only two genes, the MASP-1/3 gene encodes MASP-1 and MASP-3, whereas the MASP-2/Map19 gene gives rise to MASP-2 and the non-enzymatic MASP-2 MAp19. MASP-1 associates preferentially with low oligomer MBL (termed MBL-I), whereas MASP-2 and MASP-3 associate mainly with larger MBL oligomers (MBL II and MBL III). MASP-3 is generated by alternative splicing of the MASP-1/3 gene. The MASP-3 transcription product is composed of an A chain, which is common to both MASP-1 and MASP-3, and a B chain, which is unique to MASP- 3. The biological function of MASP-3 remains unclear. However, MASP-3 was found to downregulate the C4 and C2 cleaving activity of MASP-2. MASP-3 inhibits MASP-2 by a combination of competitive inhibition of MASP-2 association with MBL and inhibition of activation of the MBL-associated MASP-2.

UniProt: P48740

Pathways: Komplementsystem