MCM2 Antikörper

Produktnummer ABIN5542011

Der Maus-Monoklonal Anti-MCM2-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet zum Nachweis von MCM2 in Proben aus Human. }

Kurzübersicht für MCM2 Antikörper (ABIN5542011)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 4B8

Produktdetails anti-MCM2 Antikörper

Spezifität: This antibody detects ~120 kDa of MCM2 protein from whole cell lysate of human cell lines.

Kreuzreaktivität (Details): Does not react with Mouse (WR19L) and Rat (Rat1).

Aufreinigung: Protein A agarose

Immunogen: Recombinant human MCM2 protein corresponding to full-length amino acids

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1 μg/mL for chemiluminescence detection system. Immunocytochemistry: 5-10 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Details zu MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Andere Bezeichnung: mcm2

Hintergrund: In human cells, MCM4, - 6, and - 7 proteins form a stable complex, and MCM2 is loosely associated with this complex. The heterotrimeric MCM complex containing MCM4, - 6 and - 7 ha s DNA helicase activity, and such activity can be inhibited by the presence of MCM2. Cdc7/Dbf4 protein kinase, which is required for the initiation of DNA replication phosphorylates MCM2 in vitro and in vivo. From these observations, it's thought that phos phorylation of MCM2 may remove MCM2 from the MCM4, - 6, and - 7 complex to activate its DNA helicase activity at the onset of DNA replication.

UniProt: P49736

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA