NMDAR2A Antikörper (C-Term)

Produktnummer ABIN5541473

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NMDAR2A in WB und IP. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für NMDAR2A Antikörper (C-Term) (ABIN5541473)

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NMDAR2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-NMDAR2A Antikörper

Bindungsspezifität: C-Term

Spezifität: Specific for the ~180k NR2A subunit of the NMDA receptor. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Aufreinigung: Affinity purification using a column to which the fusion protein immunogen was coupled

Immunogen: Fusion protein from the C-terminus of the NR2A subunit of rat NMDA receptor.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000. Immunohistochemistry: Immunoprecipitation: 6 μL per 200 μg lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50 % glycerol

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Andere Bezeichnung: nmda receptor 2a

Hintergrund: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K /AMPAR). Those that are sensitive to N-methyl-D- aspartate (NMDA) are designated NMDA receptors (N MDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been im plicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002 Wenthold et al., 2003 Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989 Alvestad et al., 2003 Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such cha nnels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

UniProt: Q00959

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity