CEACAM5 Antikörper

Produktnummer ABIN5541180

Produktdetails anti-CEACAM5 Antikörper

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CEACAM5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC)

Spezifität: D14HD11 is a broadly reactive antibody which reacts with high affinity (affinity to solid phase bound CEACAM5: 2 x 10e10 L/Mol [6]) with most of the CEACAM molecules (except CEACAM7 and CEACAM8) transiently expressed on the cell surface of transfected BOSC23 cells. D14HD11 was included and characterized in the studies from the VIth Leucocyte Typing Workshop. It reacts with the CEACAM family N-domain.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Immunisation with extracted protein of CEACAM5

Klon: D14HD11

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1.2 μg/10 6 cells. The antibody is routinely tested on BOSC23 cells transiently transfected with a CEACAM5 expression vector. Cell based ELISA with intakt, transiently transfected cells: 1/200. ELISA: 1/200-1/400. Western blot: 4 μg/mL. Immunofluorescence: 1 μg/10 For various aplications see list of references.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.2

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-CEACAM5 Antikörper (ABIN5541180)

Kuespert, Roth, Hauck: "Neisseria meningitidis has two independent modes of recognizing its human receptor CEACAM1." in: PLoS ONE, Vol. 6, Issue 1, pp. e14609, (2011) (PubMed).

Singer, Scheffrahn, Kammerer, Suttorp, Ergun, Slevogt: "Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells." in: PLoS ONE, Vol. 5, Issue 1, pp. e8747, (2010) (PubMed).

Yue, Goldstein, Hollingsworth, Kaul, Brand, Haab: "The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1697-707, (2009) (PubMed).

Lee, Ostrowski, Gray-Owen: "CEACAM1 dynamics during neisseria gonorrhoeae suppression of CD4+ T lymphocyte activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6827-35, (2008) (PubMed).

Guignot, Hudault, Kansau, Chau, Servin: "Human decay-accelerating factor and CEACAM receptor-mediated internalization and intracellular lifestyle of Afa/Dr diffusely adhering Escherichia coli in epithelial cells." in: Infection and immunity, Vol. 77, Issue 1, pp. 517-31, (2008) (PubMed).

Schmitter, Pils, Weibel, Agerer, Peterson, Buntru, Kopp, Hauck: "Opa proteins of pathogenic neisseriae initiate Src kinase-dependent or lipid raft-mediated uptake via distinct human carcinoembryonic antigen-related cell adhesion molecule isoforms." in: Infection and immunity, Vol. 75, Issue 8, pp. 4116-26, (2007) (PubMed).

Muenzner, Rohde, Kneitz, Hauck: "CEACAM engagement by human pathogens enhances cell adhesion and counteracts bacteria-induced detachment of epithelial cells." in: The Journal of cell biology, Vol. 170, Issue 5, pp. 825-36, (2005) (PubMed).

McCaw, Liao, Gray-Owen: "Engulfment of Neisseria gonorrhoeae: revealing distinct processes of bacterial entry by individual carcinoembryonic antigen-related cellular adhesion molecule family receptors." in: Infection and immunity, Vol. 72, Issue 5, pp. 2742-52, (2004) (PubMed).

Schmitter, Agerer, Peterson, Munzner, Hauck: "Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens." in: The Journal of experimental medicine, Vol. 199, Issue 1, pp. 35-46, (2004) (PubMed).

Billker, Popp, Brinkmann, Wenig, Schneider, Caron, Meyer: "Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways." in: The EMBO journal, Vol. 21, Issue 4, pp. 560-71, (2002) (PubMed).

Boulton, Gray-Owen: "Neisserial binding to CEACAM1 arrests the activation and proliferation of CD4+ T lymphocytes." in: Nature immunology, Vol. 3, Issue 3, pp. 229-36, (2002) (PubMed).

Watt, Teixeira, Zhou, Doyonnas, Zhang, Grunert, Blumberg, Kuroki, Skubitz, Bates: "Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site." in: Blood, Vol. 98, Issue 5, pp. 1469-79, (2001) (PubMed).

Schölzel, Zimmermann, Schwarzkopf, Grunert, Rogaczewski, Thompson: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, (2000) (PubMed).

Details zu CEACAM5

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Andere Bezeichnung: cd66e,ceacam5 (CEACAM5 Produkte)

Synonyme: CD66e antikoerper, CEA antikoerper, CEACAM5 antikoerper, CEACAM antikoerper, 1600029H12Rik antikoerper, Psg30 antikoerper, carcinoembryonic antigen related cell adhesion molecule 5 antikoerper, carcinoembryonic antigen antikoerper, carcinoembryonic antigen-related cell adhesion molecule 5 antikoerper, carcinoembryonic antigen-related cell adhesion molecule 8 antikoerper, carcinoembryonic antigen gene family antikoerper, CEACAM5 antikoerper, Ceacam5 antikoerper, LOC100409241 antikoerper, Cea antikoerper

Hintergrund: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains (1,2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosylphosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumours of epithelial origin is the basis of its wide-spread use as a tumour marker (2). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumour suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).

UniProt: P06731